IL-10 antibody

Catalog No. ABIN604997

The Rat Monoclonal anti-IL-10 antibody has been validated for WB, IHC, ELISA, FACS, IHC (p) and IHC (fro). It is suitable to detect IL-10 in samples from Mouse.

Quick Overview for IL-10 antibody (ABIN604997)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: JES5-2A5

Product Details anti-IL-10 Antibody

Brand: IHC-plus™

Specificity: Recognizes mouse Cytokine synthesis inhibitory factor (CSIF), also known as interleukin-10 (IL-10). IL-10 is produced by Ly-1(CD5) expressing B cells and a subset of stimulated T cells. IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis, and the suppression of the delayed hypersensitivity response. Is reported to neutralize the activity of murine IL-10.

Purification: Protein G purified

Immunogen: Recombinant mouse IL-10.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1:10 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), WB (1:1000)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Membrane permeabilization is required for this application.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide. Sourced from tissus culture supernatant

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10

Background: Name/Gene ID: IL10
Family: Hormone

Synonyms: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF

Gene ID: 3586

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints